384 well poly-d-lysine coated flipr plates (Greiner Bio)
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Greiner Bio
384 well poly-d-lysine coated flipr plates
384 Well Poly D Lysine Coated Flipr Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/384+well+flipr+assay+plates/384+well+poly+d+lysine++pdl++coated+black+flipr+plates/us11761951-742-8-12
Average 90 stars, based on 1 article reviews
384 Well Poly D Lysine Coated Flipr Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/384+well+flipr+assay+plates/384+well+poly+d+lysine++pdl++coated+black+flipr+plates/us11761951-742-8-12
Average 90 stars, based on 1 article reviews
384 well poly-d-lysine coated flipr plates - by Bioz Stars,
2026-10
90/100 stars
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Centrifugation:Article Title: Difluoroethyl-oxazole substituted bridged spiro[2.4]heptane derivatives as ALX receptor agonists Article Snippet: The residue was purified with FC (CH2Cl2/MeOH, 9:1) to afford trans-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate as first eluting diastereomer and cis-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate as second eluting diastereomer both as colorless oils which solidified upon standing. .. They were then seeded onto Article Title: Azaindole acetic acid derivatives and their use as prostaglandin D2 receptor modulators Article Snippet: Cells are detached from culture dishes using a dissociation buffer (0.02% EDTA in PBS, Gibco) for 1 min, and collected by centrifugation at 200 g at rt for 5 min in assay buffer (equal parts of Hank's BSS (HBSS, Bioconcept) and DMEM (low glucose, without phenol red, Gibco)). .. After incubation for 45 min (37° C. and 5% CO2) in the presence of 1 μM Fluo-4 and 0.04% Pluronic F-127 (both Molecular Probes), and 20 mM HEPES (Gibco) in assay buffer, the cells are washed with and resuspended in assay buffer, then seeded onto Article Title: Piperazine substituted bridged spiro[2.4]heptane derivatives as ALX receptor agonists Article Snippet: They were then seeded onto 384-well FLIPR assay plates (Greiner, 781091) at 50′000 cells in 70 μl per well and sedimented by centrifugation at 1′000 rpm for 1 min. Stock solutions of test compounds were made up at a concentration of 10 mM in DMSO, and serially diluted in AB to concentrations required for activation dose response curves. .. They were then seeded onto Article Title: Azaindole acetic acid derivatives and their use as prostaglandin D2 receptor modulators Article Snippet: Cells are detached from culture dishes using a dissociation buffer (0.02% EDTA in PBS, Gibco) for 1 min, and collected by centrifugation at 200 g at rt for 5 min in assay buffer (equal parts of Hank's BSS (HBSS, Bioconcept) and DMEM (low glucose, without phenol red, Gibco)). .. After incubation for 45 min (37° C. and 5% CO2) in the presence of 1 μM Fluo-4 and 0.04% Pluronic F-127 (both Molecular Probes), and 20 mM HEPES (Gibco) in assay buffer, the cells are washed with and resuspended in assay buffer, then seeded onto Article Title: Azaindole acetic acid derivatives and their use as prostaglandin D Article Snippet: Cells are detached from culture dishes using a dissociation buffer (0.02% EDTA in PBS, Gibco) for 1 min, and collected by centrifugation at 200 g at rt for 5 min in assay buffer (equal parts of Hank's BSS (HBSS, Bioconcept) and DMEM (low glucose, without phenol red, Gibco)). .. After incubation for 45 min (37° C. and 5% CO2) in the presence of 1 μM Fluo-4 and 0.04% Pluronic F-127 (both Molecular Probes), and 20 mM HEPES (Gibco) in assay buffer, the cells are washed with and resuspended in assay buffer, then seeded onto Article Title: Hydroxylated aminotriazole derivatives as ALX receptor agonists Article Snippet: After 60 min incubation at 37° C. under 5% CO2 in AB supplemented with 1 μM Fluo-4 (AM) (Invitrogen, F14202) and 20 mM HEPES (Gibco, 15630-056), the cells were washed and resuspended in AB. .. They were then seeded onto Concentration Assay:Article Title: Difluoroethyl-oxazole substituted bridged spiro[2.4]heptane derivatives as ALX receptor agonists Article Snippet: The residue was purified with FC (CH2Cl2/MeOH, 9:1) to afford trans-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate as first eluting diastereomer and cis-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate as second eluting diastereomer both as colorless oils which solidified upon standing. .. They were then seeded onto Article Title: Piperazine substituted bridged spiro[2.4]heptane derivatives as ALX receptor agonists Article Snippet: They were then seeded onto 384-well FLIPR assay plates (Greiner, 781091) at 50′000 cells in 70 μl per well and sedimented by centrifugation at 1′000 rpm for 1 min. Stock solutions of test compounds were made up at a concentration of 10 mM in DMSO, and serially diluted in AB to concentrations required for activation dose response curves. .. They were then seeded onto Article Title: Hydroxylated aminotriazole derivatives as ALX receptor agonists Article Snippet: After 60 min incubation at 37° C. under 5% CO2 in AB supplemented with 1 μM Fluo-4 (AM) (Invitrogen, F14202) and 20 mM HEPES (Gibco, 15630-056), the cells were washed and resuspended in AB. .. They were then seeded onto Activation Assay:Article Title: Difluoroethyl-oxazole substituted bridged spiro[2.4]heptane derivatives as ALX receptor agonists Article Snippet: The residue was purified with FC (CH2Cl2/MeOH, 9:1) to afford trans-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate as first eluting diastereomer and cis-tert-butyl 4-amino-3-fluoropiperidine-1-carboxylate as second eluting diastereomer both as colorless oils which solidified upon standing. .. They were then seeded onto Article Title: Piperazine substituted bridged spiro[2.4]heptane derivatives as ALX receptor agonists Article Snippet: They were then seeded onto 384-well FLIPR assay plates (Greiner, 781091) at 50′000 cells in 70 μl per well and sedimented by centrifugation at 1′000 rpm for 1 min. Stock solutions of test compounds were made up at a concentration of 10 mM in DMSO, and serially diluted in AB to concentrations required for activation dose response curves. .. They were then seeded onto Article Title: Hydroxylated aminotriazole derivatives as ALX receptor agonists Article Snippet: After 60 min incubation at 37° C. under 5% CO2 in AB supplemented with 1 μM Fluo-4 (AM) (Invitrogen, F14202) and 20 mM HEPES (Gibco, 15630-056), the cells were washed and resuspended in AB. .. They were then seeded onto Incubation:Article Title: Azaindole acetic acid derivatives and their use as prostaglandin D2 receptor modulators Article Snippet: Cells are detached from culture dishes using a dissociation buffer (0.02% EDTA in PBS, Gibco) for 1 min, and collected by centrifugation at 200 g at rt for 5 min in assay buffer (equal parts of Hank's BSS (HBSS, Bioconcept) and DMEM (low glucose, without phenol red, Gibco)). .. After incubation for 45 min (37° C. and 5% CO2) in the presence of 1 μM Fluo-4 and 0.04% Pluronic F-127 (both Molecular Probes), and 20 mM HEPES (Gibco) in assay buffer, the cells are washed with and resuspended in assay buffer, then seeded onto Article Title: Azaindole acetic acid derivatives and their use as prostaglandin D2 receptor modulators Article Snippet: Cells are detached from culture dishes using a dissociation buffer (0.02% EDTA in PBS, Gibco) for 1 min, and collected by centrifugation at 200 g at rt for 5 min in assay buffer (equal parts of Hank's BSS (HBSS, Bioconcept) and DMEM (low glucose, without phenol red, Gibco)). .. After incubation for 45 min (37° C. and 5% CO2) in the presence of 1 μM Fluo-4 and 0.04% Pluronic F-127 (both Molecular Probes), and 20 mM HEPES (Gibco) in assay buffer, the cells are washed with and resuspended in assay buffer, then seeded onto Article Title: Azaindole acetic acid derivatives and their use as prostaglandin D Article Snippet: Cells are detached from culture dishes using a dissociation buffer (0.02% EDTA in PBS, Gibco) for 1 min, and collected by centrifugation at 200 g at rt for 5 min in assay buffer (equal parts of Hank's BSS (HBSS, Bioconcept) and DMEM (low glucose, without phenol red, Gibco)). .. After incubation for 45 min (37° C. and 5% CO2) in the presence of 1 μM Fluo-4 and 0.04% Pluronic F-127 (both Molecular Probes), and 20 mM HEPES (Gibco) in assay buffer, the cells are washed with and resuspended in assay buffer, then seeded onto other:Article Title: Benzimidazolyl-methyl urea derivatives as ALX receptor agonists Article Snippet: After 60 min incubation at 37° C. under 5% CO2 in AB supplemented with 1 μM Fluo-4 (AM) (Invitrogen, F14202) and 20 mM HEPES (Gibco, 15630-056), the cells were washed and resuspended in AB. |